Fig. 6.
Effect of cell density on cytoskeletal proteins. Immunofluorescence staining (Alexa Fluor 488 secondary antibody) with DAPI nuclear staining showing a fibronectin, b nestin and c vimentin staining of high density (seeded at 5 × 104 cells/cm2) hDPSCs. Low density (seeded at 5 × 103 cells/cm2) hDPSCs were also stained with d fibronectin, e nestin and f vimentin. Fibronectin appears to be upregulated as hDPSCs increase in density whereas nestin production is reduced. Vimentin production is unaffected by cell density. Scale bar 100 μm