Table 1.
List of antibodies and reagents used in immunohistochemical investigations
| Antigen | Code | Species | Dilution/concentration | Supplier |
|---|---|---|---|---|
| Primary antibodies | ||||
| 1 | 2 | 3 | 4 | 5 |
| CART | H-003-61 | Rabbit | 1:20000 | Phoenix, Pharmaceuticals INC, Belmont, CA, USA |
| GAL | RIN7153 | Rabbit | 1:4000 | Peninsula, San Carlos, CA, USA |
| nNOS | N2280 | Mouse | 1:1000 | Sigma-Aldrich, Saint Luis, MS, USA |
| PACAP-27 | IHC 8922 | Rabbit | 1:20000 | Phoenix Pharmaceuticals, INC, Belmont, CA, USA |
| SP | 8450-0505 | Rat | 1:500 | Biogenesis Ltd., Poole, England |
| VIP | VA1285 | Rabbit | 1:6000 | Biomol, Hamburg, Germany |
| Secondary antibodies and complexes of fluorochromes | ||||
| 1 | 4 | 5 | ||
| Alexa fluor 488 donkey anti-mouse IgG | 1:1000 | Invitrogen, Carlsbad, CA, USA | ||
| Alexa fluor 488 donkey anti-rat IgG | 1:1000 | Invitrogen | ||
| Alexa fluor 546 donkey anti-rabbit IgG | 1:1000 | Invitrogen | ||
| Antigens used in pre-absorption tests | ||||
| 1 | 3 | 4 (μM) | 5 | |
| CART | C5977 | 0.1 | Sigma, St. Louis, MO, USA | |
| GAL | G0278 | 0.5 | Sigma | |
| NOS | N3033 | 1.0 | Sigma | |
| PACAP-27 | 052-02 | 0.3 | Phoenix Pharmaceuticals | |
| SP | S6883 | 0.7 | Sigma | |
| VIP | V6130 | 1.0 | Sigma | |