Figure 3.

Presence of full-length A1M in normal NMRI mice in kidneys and serum at 10, 20 and 60 minutes p.i. Animals were injected i.v. with 150 μg A1M and blood and kidneys collected at the indicated time-points. The blood was allowed to coagulate and serum separated by centrifugation. One kidney was homogenized in 1 ml PBS and centrifugated. One μl serum and 6 μl supernatant from the kidney homogenate were applied to SDS-PAGE, transferred to PVDF-membranes and blotted with anti-A1M. Each lane represents a separate mouse.