Table 1.
Gene name | Primer | Annealing temperature (°C) | Products size (bp) | |
---|---|---|---|---|
Fascin | Sense | 5’-CTGGCTACACGCTGGAGTTC-3’ | 62 | 492 |
Antisense | 5’-CTGAGTCCCCTGCTGTCTCC-3’ | |||
β-actin | Sense | 5’-TGACGTGGACATCCGCAAAG-3’ | 58 | 205 |
Antisense | 5’-CTGGAAGGTGGACAGCGAGG-3’ | |||
Smad3 | Sense | 5’-CGCTTGACTCACGCCTTCG-3’ | 62 | 422 |
Antisense | 5’-TTCCTCTTGCGGCCACTT-3’ |