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. 2015 May 15;5(6):1890–1896.

Table 1.

Sequences of primers used for PCR

Gene name Primer Annealing temperature (°C) Products size (bp)
Fascin Sense 5’-CTGGCTACACGCTGGAGTTC-3’ 62 492
Antisense 5’-CTGAGTCCCCTGCTGTCTCC-3’
β-actin Sense 5’-TGACGTGGACATCCGCAAAG-3’ 58 205
Antisense 5’-CTGGAAGGTGGACAGCGAGG-3’
Smad3 Sense 5’-CGCTTGACTCACGCCTTCG-3’ 62 422
Antisense 5’-TTCCTCTTGCGGCCACTT-3’