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Silencing of Wnt10B attenuates the proliferation in HepG2 cells in culture. HepG2 cells transfected with pLVTHM (control), pLV-shwnt10b1 (shWnt10b#1), or pLV-shwnt10b2 (shWnt10b#2) were cultured for 2 to 92 hours and at different time points assessed with a CCK-assay to determine cell proliferation rate (absorbance at 450 nm). Results are expressed as the mean ± SD (n=3); *P<0.05, **P<0.01, vs. control; #P<0.05, vs. shWnt10b#1.
