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Silencing of Wnt10B delays the colony formation in HepG2 cells in culture. HepG2 cells transfected with pLVTHM (control), pLV-shwnt10b1 (shWnt10b#1), or pLV-shwnt10b2 (shWnt10b#2) were cultured for 2-3 weeks, then cell growth and colony formation determined. Photos: a representative result from three independent experiments. Bar graph: data are shown as mean ± SD (n=3). *P<0.05, **P<0.01 vs. control; #P<0.05, vs. shWnt10b#1.
