Figure 5.

Silencing of Wnt10B obstructs migration in HepG2 cells in culture. HepG2 cells transfected with pLVTHM (control), pLV-shwnt10b1 (shWnt10b#1), or pLV-shwnt10b2 (shWnt10b#2) were cultured to reach to confluence, scratched and cultured continually for 48-96 hours, then the cell migration and wound healing assessed by microscopy at different time points. Photos: a representative result from three independent experiments. Bar graph: data are shown as mean ± SD (n=3). *P<0.05, **P<0.01 vs. control; #P<0.05, vs. shWnt10b#1.