Figure 2.

Identification of the miR-544 promoter. A. Agarose gel electrophoresis shows the PCR products of 5’RACE. B. Schematic of the miR-544 promoter location in the genome. The transcription start site of miR-544 is indicated as +1 and the number indicates the relative distance from the miR-544 transcription start site. C. Alignment of the miR-544 promoter sequence between different species. D. Relative luciferase activity of the miR-544 promoter. Luciferase constructs containing the miR-544 promoter (pGL3-P 1 to P 5) and the unmodified construct (pRL-TK) were co-transfected into HeLa cells. Firefly luciferase activity was normalized to Renilla luciferase activity. Data represent the mean ± SD from three independent experiments. *P < 0.05.