Table 1.
Primer sequences used in this study
| Primer name | primer sequences |
|---|---|
| RT-miR-544 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACgaactt |
| RT-RNU6B | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAAATAT |
| qRT-miR-544-F | gccgcgATTCTGCATTTTTAGC |
| qRT-RNU6B-F | TTCCTCCGCAAGGATGACACGC |
| qRT-miRNA-R | GTGCAGGGTCCGAGGT |
| YWHAZ 3’UTR-1-F-XhoI | ccgctcgagGGATACCCAAGGAGACGA |
| YWHAZ 3’UTR-1-R-NotI | ataagaatgcggccgcCTCAAGTTATTTCCCTGTTA |
| YWHAZ 3’UTR-2-F-XhoI | ccgctcgagTGACATTGGGTAGCATTA |
| YWHAZ 3’UTR-2-R-NotI | ataagaatgcggccgcGTTTGGGATTTCAGTTTCT |
| 1YWHAZ 3’UTR-mut-F1 | TTGACTACAGCTTGCCGAGTGTTCCTTTA |
| 1YWHAZ 3’UTR-mut-R1 | TAAAGGAACACTCGGCAAGCTGTAGTCAA |
| 1YWHAZ 3’UTR-del-F1 | ACCTTTGACTACAGCGTGTTCCTTTAGAC |
| 1YWHAZ 3’UTR-del-R1 | GTCTAAAGGAACACGCTGTAGTCAAAGGT |
| 1YWHAZ 3’UTR-mut-F2 | CTCTGGATAAGGGAGCCAACGGTTCACATT |
| 1YWHAZ 3’UTR-mut-R2 | AATGTGAACCGTTGGCTCCCTTATCCAGAG |
| 1YWHAZ 3’UTR-del-F2 | TTACTCTGGATAAGGACGGTTCACATTC |
| 1YWHAZ 3’UTR-del-R2 | GAATGTGAACCGTCCTTATCCAGAGTAA |
| 2YWHAZ 3’UTR-mut-F | CCTCAGTACTTTAGCCGAAACACCAAACA |
| 2YWHAZ 3’UTR-mut-R | TGTTTGGTGTTTCGGCTAAAGTACTGAGG |
| 2YWHAZ 3’UTR-del-F | TTCCTCAGTACTTAACACCAAACA |
| 2YWHAZ 3’UTR-del-R | TGTTTGGTGTTAAGTACTGAGGAA |
| qRT-YWHAZ-F | CCTCACTCCCGTTTCCG |
| qRT-YWHAZ-R | CAGCACCTTCCGTCTTT |
| qRT-GAPDH-F | ATGGGGAAGGTGAAGGTCG |
| qRT-GAPDH-R | CTGGAAGATGGTGATGGGA |
| miR-544 5’RACE outer | ACACAAAACGGATACACACGGTCA |
| miR-544 5’RACE inner | TGGGTCCAAGACAAGGTTTGATAC |
| miR-544 promoter (-255)-F-XhoI | ccgctcgagGGCTCTGTGAGATGGATGA |
| miR-544 promoter (+15)-F-XhoI | ccgctcgagAGGCTGATTCTGGTGAAGG |
| miR-544 promoter (+256)-F-XhoI | ccgctcgagTTCGGTTTATTGACATGGA |
| miR-544 promoter (+764)-R-HindIII | cccaagcttAAAGGTTAGCACGGAAAAG |
| miR-544 promoter (+918)-R-HindIII | cccaagcttCAAGGTTGGACGGTTAGTA |
| YWHAZ-F-EcoRI | ccggaattcCCACCATGGATAAAAATGAGCTGG |
| YWHAZ-R-KpnI | cggggtaccTTAATTTTCCCCTCCTTCTCC |
| KLF4-F-XhoI | ccgctcgagCCACCATGAGGCAGCCACCTGG |
| KLF4-R-HindIII | cccaagcttTTAAAAATGCCTCTTCATGTGT |
| qRT-KLF4-F | GCCAAAGAGGGGAAGACGAT |
| qRT-KLF4-R | CGCAGGTGTGCCTTGAGATG |
| ChIP qRT-F | ACCTGCCCAGTGCTATTGTTT |
| ChIP qRT-R | CAAAACGGATACACACGGTCA |
| KLF4-miR-544-mut-F1 | ACTCAGCAAGAAGCACGGTATGTGT |
| KLF4-miR-544-mut-R1 | ACACATACCGTGCTTCTTGCTGAGT |
| KLF4-miR-544-mut-F2 | ATGACTTTAGAAGGAATGATGGCCTCTGG |
| KLF4-miR-544-mut-R2 | CCAGAGGCCATCATTCCTTCTAAAGTCAT |
| KLF4-miR-544-mut-F3 | GCCTCTGGGATTATTCCTCTCCTGG |
| KLF4-miR-544-mut-R3 | CCAGGAGAGGAATAATCCCAGAGGC |
| KLF4-miR-544-mut-F4 | GGTGCAGTGTAAATAAGGCCAAAAGC |
| KLF4-miR-544-mut-R4 | GCTTTTGGCCTTATTTACACTGCACC |
| KLF4-miR-544-mut-F5 | TGGGTCCCTGTAATTTGAGCGGGAA |
| KLF4-miR-544-mut-R5 | TTCCCGCTCAAATTACAGGGACCCA |
| KLF4-miR-544-mut-F6 | TTCCTGAGCGAATTATCATCAGCCC |
| KLF4-miR-544-mut-R6 | GGGCTGATGATAATTCGCTCAGGAA |
| KLF4-miR-544-mut-F7 | CGATGGCTGGAACAACGCTCGTGTG |
| KLF4-miR-544-mut-R7 | CACACGAGCGTTGTTCCAGCCATCG |
| KLF4-miR-544-mut-F8 | GCATCTAATTTGCTTCCAGGACCGC |
| KLF4-miR-544-mut-R8 | GCGGTCCTGGAAGCAAATTAGATGC |
| KLF4-miR-544-mut-F9 | CGTACCAAGTTTGTTCAAGGCAGTG |
| KLF4-miR-544-mut-R9 | CACTGCCTTGAACAAACTTGGTACG |
Mutant sequences were underlined.