Figure 5.

RARRES3 interacted with MTDH in an opposite way. A. Expression variation of MTDH, β-catenin, Fibronectin, E-cadherin after ectopic expression of RARRES3 in HT-29 and SW-620 where basal expression of RARRES3 was highest and lowest respectively; B. Expression of β-catenin both in cytoplasmic and nuclear compartment was detected using western-blotting; GAPDH and Histone H4 were as loading control. 100 μg total protein was loaded per lane, which was separated by 10% SDS-PAGE followed by visualization with Western Breeze kit (Invitrogen, USA). C. Immunoprecipitation analysis of RARRES3 and MTDH. 293T cells transfected with the eukaryotic expression vector of RARRES3 that contains Flag tag on N-terminus were harvested for immunoprecipitation (IP) using anti-FLAG M2-agarose beads. FLAG precipitates were subjected for immunoblot using anti-RARRES3, anti-MTDH, or anti-GAPDH antibody.