Figure 2.
Overexpressed or silenced Nodal affect tumorigenic capacity and tumor metastasis in vivo. C57BL/6 mice were transplanted with 4 × 105 of each B16 cell lines cells respectively with i.p. injection. A. Representative macroscopic appearance of each B16 cell lines allograft. B. Values represent the median tumor volume. C. H&E examination of each B16 cell lines metastasis in lung and liver tissue section. Scale bar, 50 m. D. The tumor tissue sections were subjected to IHC detection of Nodal, E-cadherin and vimentin. Scale bar, 50 m.