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. 2015 May 15;5(6):2098–2112.

Figure 4.

Figure 4

Nodal up-regulates Snail and Slug partly via activation of ALK/Smads pathway and PI3k/AKT pathway. A. The protein of B16 cells, B16-pldNodal cells and B16-shNodal cells were collected for Western blotting analysis to test the activity of ALK pathway and PI3k/AKT pathway. B. B16-pldMock cells and B16-pldNodal cells were treating with SB431542 (10 mM) in a time dependent manner, and then the protein was collected for Western blotting analysis. C. B16-pldMock cells and B16-pldNodal cells were treat with LY2940002 (10 mM) in a time dependent manner, and then the protein was collected for Western blotting analysis. D. B16-pldNodal cells and B16-pldMock cells were treated with SB431542 (10 mM) or LY29004 (10 mM) overnight, controlled by DMSO, and then subjected to wound healing scratch assay. Cells were accompanied by SB431542 or LY29004 treatment over the experiment. Scale bar, 50 m. E. B16-pldNodal cells were treated with SB431542 (10 mM) or EGF (100 ng/ml) for 24 h, and then the protein was collected for Western blotting analysis.