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. 2015 May 15;5(6):2098–2112.

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EMT is not completely inhibited by blocking the paracrine of Nodal. A. B16, B16-pldNodal cells and B16-pldMock cells were treated with Nodal neutralizing antibody (rabbit polyclonal antibody) overnight, controlled by rabbit serum, and then subjected to wound healing scratch assay. Cells were accompanied by Nodal neutralizing antibody over the experiment. B. B16-pldNodal cells and B16-shNodal cells were 1:1 mixed, and co-cultured for 28 days and then mixed cells were observed by fluorescence microscope. B16-pldNodal emit red light and B16-shNodal emit green light. C. The mixed cells were subjected for wound healing scratch assay. D. The mixed cells were treated with SB431542 (10 mM) or LY29004 (10 mM) overnight, controlled by DMSO, and then subjected to wound healing scratch assay. Cells were accompanied by SB431542 or LY29004 treatment over the experiment. Scale bar: 50 m in A-D.