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. 2015 May 27;6(1):108. doi: 10.1186/s13287-015-0101-6

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© Gálvez-Montón et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Neoinnervation of pericardial-derived scaffolds after myocardial infarction. Histological sections of the pericardial-derived scaffold showing nerve fibers (arrows) after Gallego’s modified trichrome (a), light green Masson’s trichrome (b), and hematoxylin/eosin (c) staining. d-d′′ Immunohistological analysis exhibiting positive S100 nerve structures in the scaffold and corresponding zoomed images. e and f Immunofluorescence against β_III tubulin (green), cardiac troponin I (white), and elastin (red) labeled nerve fibers (arrows), myocardium (M) and pericardial-derived bioimplant (P), respectively. Nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue) (g and h). Transmission electron microscopy images of the scaffold showing nerve structures composed of Schwann cells (S), axons (A), and fibroblasts of the perineurium (F), endoneurium (E), transport vesicles (TV), microtubules (M), mitochondria (MT), and synaptic vesicles (SV). Scale bars = 100 μm (d), 50 μm (a, b, d′, e, and f), 20 μm (c and d′′), 5 μm (g), and 2 μm (h)