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. 2015 May 27;6(1):108. doi: 10.1186/s13287-015-0101-6

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© Gálvez-Montón et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Neovascularization of pericardial-derived scaffolds after myocardial infarction. a Masson’s trichrome staining composition screening demonstrated the correct adhesion of the cardiac bioimplant with the adjacent myocardium. b and c-c′′ Gallego’s modified trichrome and hematoxylin/eosin staining exhibiting vessels in the cardiac matrix and zoomed images, respectively. Immunofluorescence analysis in the pericardial-derived scaffold after its implantation against isolectin B4 (green), smooth muscle actin (red), and elastin (white) indicates the presence of microvasculature (d), venules (e), and arterioles (f). Red positive immunostaining against CD31 (g) and von Willebrand factor (h) antibodies in pericardial-derived scaffold (arrows). Nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue) (i and j). Transmission electron microscopy images of the pericardial-derived scaffold show vascular structures (arrows) where endothelial cells (EC), pericytes (PC), and erythrocytes (E) are present. M myocardium, P pericardial-derived scaffold. Scale bars = 100 μm (a-c), 50 μm (c′ and d-h), 20 μm (c′′), and 5 μm (i and j)