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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Acta Biomater. 2014 Aug 13;10(11):4704–4714. doi: 10.1016/j.actbio.2014.08.007

Figure 4. Polymer release of FTY720 generates a local in vivo gradient.

Figure 4

Tissue biopsies of 1.5 and 4mm diameter centered over the origin of the film (A) were analyzed by HPLC-MS/MS for lipid species of 3 days after surgery and film implantation. NBD-FTY720 film shows release of fluorescent FTY720 in a gradient from the edge of the film outward in vivo (A). FTY720 films release more total compound (white=FTY720, black=FTY720-P) in both the 1.5mm (B) and 4mm (C) tissue regions in vivo. Concentrations of FTY720 (D) and FTY720-P (E) in the tissue at varying distances from the film demonstrate differential gradients between the FTY720 and FTY720-P films. FTY720 and FTY720-P release at different rates in vitro (F). Sphingosine (G) and S1P (H) were measured in the tissue segments illustrated in A. Change in arteriolar diameter with FTY720 or FTY720-P 3 days after surgery (I; n=5–8). Representative images of vascular changes from day 0 to day 3, white arrows indicate arterioles (J). (*p<0.05 compared to FTY720 film)