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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Dev Biol. 2015 Jun 17;405(1):56–70. doi: 10.1016/j.ydbio.2015.06.009

Figure 4. Epithelial Wls is required for prechondrogenic mesenchymal condensations.

Figure 4

Tracheal lung explants of E13.5 embryos were stained with PNA lectin. Lack of staining demonstrates absence of mesenchymal condensations in tracheas of Wlsf/f;ShhCre/+ embryos (arrow B vs. arrow A). Insets in A and B correspond to bright field images of whole mounts stained with PNA lectin. Confocal images of E13.5 whole mounts show decreased Sox9 staining in tracheal mesenchyme (arrow in C, and D). Sox9 staining in cells of the peripheral lung overlapped with Nkx2.1 in both control and Wlsf/f;ShhCre/+ pulmonary tissue (arrowhead in G and H). Es= esophagus, Br=Bronchus, Sp=spine.