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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Dev Biol. 2015 Jun 17;405(1):56–70. doi: 10.1016/j.ydbio.2015.06.009

Figure 7. Canonical Wnt signaling modulates expression of Sox9 ex vivo and in vitro.

Figure 7

Bmp-2 (100ng/ml) induced Sox9 expression in Wlsf/f;ShhCre/+ tracheal explants, while Wnt3a or Wnt5a (200 ng/ml) did not induce Sox9 in tracheal explants. (A) Addition of Wnt3a and Wnt5a to culture media of Wlsf/f;ShhCre/+ explants increased Sox9 and Col2a1 mRNA (B). Control tracheal explants were cultured in the presence of the canonical Wnt antagonist XAV-939. Addition of XAV-939 (1uM) reduced expression of Col2a1, Lef1 (a direct canonical Wnt target) and to lesser degree Sox9 (C). In MEF micromass cultures, addition of Bmp-2 robustly induced Sox9 and Col2a1. Addition of Bmp-2 and LiCl repressed Bmp-2 induced Sox9 in MEF micromasses (D). N=5 * p<0.05 ** p<0.02 vs. C.