PHTs were cultured in DMEM with glucose or PJ in 20% O2 for 8 h and then subjected to ≤1% O2 for 16 h or cultured initially in 20% O2 for 20 h and then exposed to CoCl2 for 4 h. (A, D) Representative Western blots of NDRG1 and cl-Parp in PHTs exposed to hypoxia (A) or CoCl2 (D). (B, C, E, F) Quantification of NDRG1 (B, E) and cl-Parp (C, F) in PHTs exposed to hypoxia (B, C) or cobalt chloride (E, F), as represented in A and D. (n=4 PHTs and n=3 BeWo experiments, *P ≤ 0.05 by Student's t-test).