Figure 3. Effect of knockdown of NDRG1 on apoptosis in BeWo cells subjected to hypoxia in the presence of PJ or glucose.
BeWo cells were incubated for 24 h in 20% O2 with siNDRG1 or scrambled siRNA control. Cells were then pretreated in DMEM with either PJ or glucose in 20% O2 for 8 h and then exposed to ≤1% O2 for 16 h while continuing PJ or glucose treatment. (A) Representative Western blots for NDRG1 in transfected BeWo cells. (B, C) Quantification of NDRG1 (B) and cl-Parp (C), as represented in A. (n=3 experiments, *P ≤ 0.05 by Student's t-test).