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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2015 May 29;14(8):1896–1906. doi: 10.1158/1535-7163.MCT-14-0865

Figure 1. The TMPRSS2/ERG fusion gene induces DNA damage in VCaP cells.

Figure 1

Confocal immunostaining for γH2AX (A) and 53BP1 (B) at the indicated times following 10 Gy ionizing radiation (IR) in VCaP parental and siERG-expressing derivative cells. C, Quantification of the number of IR-induced foci (IRIFs) in VCaP parental and derivative cells with ERG depleted by siERG. Left top panel represents western blot for levels of ERG in control (Ct) and siERG-treated VCaP cells. Typically, > 70 cells, representing different fields, were scored for IRIFs. β-actin was used as a loading control. Error bars represent SD (n=3). P < 0.0001 was calculated by two-way ANOVA.