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. Author manuscript; available in PMC: 2015 Aug 8.
Published in final edited form as: J Proteome Res. 2015 Jul 14;14(8):3403–3408. doi: 10.1021/acs.jproteome.5b00547

Fig. 2.

Fig. 2

Native proteome extraction from diverse mammalian cell types using focused acoustic sonication of cells lysed in admixture of poloxamer and octyl glucoside. (a) Unique proteins and (b) unique peptides identified. (c) Modified peptides identified in unfractionated lysates of cervical carcinoma (HeLa S3) cells, retinal pigment epithelium (RPE), acute myeloid leukemia (OCI-AML2) cells, lysed in the mixture of 1% poloxamer and octyl glucoside (Pluronic + OBG, red) as compared to 1% SDS (SDS, black), 6 M guanidine hydrochloride (GdmCl, purple) and RapiGest (RapiGest, pink). (d) Venn diagram showing the overlap of unique proteins identified from cells, lysed in the mixture of 1% poloxamer and octyl glucoside (Pluronic + OBG, red) as compared to 1% SDS (SDS, black). Error bars represent standard deviations of three technical replicates.

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