Fig. 2.

Morpholino oligonucleotide knockdown of NTPDase2 (NTPD2MO) or the P2Y1 receptor (P2Y1MO) alters the expression of Pax6 and Rx1 (a–h). The morpholino constructs were injected on just one side of the early embryo to facilitate a comparison with the uninjected side as an internal control. NTPD2MO either on its own or in combination with P2Y1MO reduced Pax6 and Rx1 expression only on the injected side (arrows). However, injection of P2Y1MO on its own or a control morpholino (CMO) has no effect on expression of Pax6 and Rx1 (i–k). This reduced expression of Pax6 and Rx1 results in smaller eyes on the injected side (indicated by lacZ staining) or, in the case of the dual morpholino knockdown, no eyes (k, arrow) (reproduced with permission from [26])