Fig. 1.

EGb761 ameliorated the effects of TNF-α on the expression of MMP-1, MMP-3, MMP-13, TIMP-1, and TIMP-2 at mRNA levels. Cells were pretreated with EGb761 at the concentration of 0, 100, 250, and 500 μg/ml for 24 h followed by treatment with TNF-α (10 ng/ml) for another 24 h. mRNA was used isolated and used for real-time PCR analysis. a MMP-1; b MMP-3; c MMP-13; d TIMP-1; e TIMP-2. (*P < 0.001 compared to untreated control; #P < 0.001 compared to TNF-α treated group)