Fig. 4.

EGb761 ameliorated TNF-α induced activation of NF-κB pathway. Cells were pretreated with EGb761 at the concentration of 0, 100, 250, and 500 μg/ml for 24 h followed by treatment with TNF-α (10 ng/ml) for another 24 h. a Phosphorylation of IKK, IκBα, and p65; b nuclear levels of p65. Lamin B was used for internal control; c luciferase activity of NF-κB (*P < 0.001 compared to untreated control; #P < 0.001 compared to TNF-α treated group)