Figure 2.

AcE and Qt are not toxic to RANKL/PgLPS-induced RAW 264.7 cell. Cells were cultured in the presence of PgLPS, RANKL, and AcE or Qt. Negative control (Ctrl) cells were not exposed to AcE or Qt. After 5 days of continuous exposure, cell viability was assessed using alamarBlue assay (a) and total protein levels were assessed using protein assays (b). There was no significant difference between control (Ctrl) and cells in all concentrations tested, ANOVA-Tukey test. Results were expressed in mean ± SD of three independent experiments.