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. 2015 Jul 26;2015:704781. doi: 10.1155/2015/704781

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Copyright © 2015 Tatiane Oliveira et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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RANKL/PgLPS-induced RAW 264.7 cells were affected by AcE or Qt during osteoclastogenesis. (a) TRAP staining by brightfield microscope showed differentiated osteoclasts in the presence of AcE or Qt after 5 days. The arrows show decreased size of osteoclasts; TRAP-stained, fixed cultures were for number of nuclei per cell in two different groups: (b) 2–5 small and (c) +10 large nuclei per cell. (d) The acid phosphatase activity of the osteoclasts was decreased in all AcE and Qt concentrations tested: ^∗ p < 0.05, ^∗∗ p < 0.01, and ^∗∗∗ p < 0.001 when compared with Ctrl group according to the ANOVA and Tukey tests. Results were expressed in mean ± SD of three independent experiments.