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. Author manuscript; available in PMC: 2016 Aug 6.
Published in final edited form as: Mol Cell. 2015 Aug 6;59(3):478–490. doi: 10.1016/j.molcel.2015.07.009

Figure 6.

Figure 6

Dominant negative effects of mutant RAD51 T131P on WT RAD51 activities in vitro. (A) DNA strand exchange by WT RAD51 in the presence of increasing amounts of RAD51 T131P. (B) Joint molecule formation by RAD51 WT and T131P pre-mixed at different ratios. (C) DNA strand exchange by RAD51 WT and T131P in the presence of BRCA2. Schematic of DNA strand exchange is shown above the gel and the quantification of DNA strand exchange products is shown to the right of the gel. RAD51 WT and T131P were pre-mixed at different ratios and added to RPA-coated ssDNA together with BRCA2. Error bars: s.d. (n=3). Also see Figure S7.