Fig. 1.

Strategy for data mining and candidate gene identification. Step 1: C3A cell cultures treated with LPON, OLE or untreated controls, as described under ‘Methods’, were subject to transcriptomic or proteomic analysis and data screened for candidate genes specifically up-regulated by nutrient overload using the screening criteria outlined in Box 1. Step 2: Predicted functional partners of primary candidates showing >2.0 fold nutrient overload-induced increases in gene transcripts and protein abundance were identified by STRING network associations. Step 3: Primary gene candidates, identified in step 1, and their predicted functional partners, identified in step 2, were grouped by enrichment analysis according to their functional annotations to identify representation of common biological processes among the candidate genes. Further details of these bioinformatics procedures are described under ‘Methods’.