Figure 2.
The results of the GDNF expression by multiplex bead immunoassay in supernatants of GDNF transduced PHCEC in vitro (A) and in the supernatants of bioengineered human corneal epithelial constructs (B). The GDNF concentration was significantly higher in the GDNF transduction group (Dunnett's test, n = 3, *, P < 0.05; **, P < 0.01, compared with undetectable levels in the non-transduction group at all time points). There was no significant difference observed between the non-transduction group and the reporter gene eGFP transduction groups at the determined time point both in vitro and ex vivo (Dunnett's test, P > 0.05).