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Immunofluorescent staining for GDNF in the bioengineered human corneal construct at day 5 after autograft ex vivo. Propidium iodide (PI) was used as nuclear counterstaining (red). There were 2–6 layers of epithelial cells covering the stroma of the graft (red). GDNF was expressed by all layers of the epithelial cells (green) in the GDNF transduction group (GDNF). There was no GDNF expression in the epithelium in the non-transduction group (control). Scale bars, 25 μm.
