FIGURE 3.

Comparison of virus persistence and AKT phosphorylation in WT and CD4^−/− mice. (A) WT and CD4^−/− mice were infected intranasally with 100 pfu of WSN-SIY virus. BAL was harvested at the indicated dpi and virus titer measured by plaque assays. ND, not detected. (B–G) Naive 2C T cells were adoptively transferred into WT and CD4^−/− mice followed by intranasal infection with WSN-SIY. At 7 and 9 dpi, cells from the lung and spleen were stained for CD8, 2C TCR, CD27 and pAKT308. Shown are CD27 vs. pAKT308 staining profiles gating on CD8⁺ 2C TCR⁺ cells at 7 dpi (B) and 9 dpi (E), percentages of CD27^hi pAKT308⁺ 2C cells at 7 dpi (C) and 9 dpi (F), and MFI of pAKT308 of CD27^hi 2C cells at 7 dpi (D) and 9 dpi (G). (H) Naïve OTI and 2C cells were isolated from OTI rag1^−/− Thy1.1⁺ and 2C rag1^−/− Thy1.2⁺ mice, respectively. These cells were mixed at the 1 to 1 ratio and activated by either SIY or SIINFEKL peptides. Two days later, cells were stained for Thy1.1, Thy1.2, CD8 plus CD69 or pAKT308 or pAKT473. Shown are Thy1.1 vs. CD69 or pAKT308 or pAKT473 staining profiles gating on CD8⁺ cells. Error bars: SEM from 4 mice per group in one of two experiments. * P<0.05.