Skip to main content
. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: J Immunol. 2015 Jul 1;195(4):1868–1882. doi: 10.4049/jimmunol.1402243

Figure 2. Verticillin A is a selective HMTase inhibitor.

Figure 2

A. Verticillin A selectively inhibits SUV39H1, SUV39H2, G9a and GLP in vitro. Verticillin A was tested in a 10-dose IC50 mode with 3-fold serial dilutions using 3H-S-adenosyl-methionine as substrate with recombinant human HMTases as indicated. The enzyme activity was then analyzed and plotted against verticillin A concentrations. IC50s were calculated using the Graphpad Prism program. Assays were performed three times with three lots of verticillin A. Shown are representative results of one of three independent assays. B. Verticillin A inhibits H3K9 methylation in human CRC cells. Metastatic human CRC LS411N and SW620 cells were treated with verticillin A at the indicated concentrations for 2 days. Histone acidic extracts were prepared and analyzed by Western blot with antibodies specific for the indicated methylated lysines of histone 3. The blot was stripped and reprobed with anti-H3 antibody as a loading control.