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. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: J Immunol. 2015 Jul 1;195(4):1868–1882. doi: 10.4049/jimmunol.1402243

Figure 5. Verticillin A treatment decreases H3K9me3 in the FAS promoter region.

Figure 5

A. Left Panel: SW620 cells were treated with verticillin A (50 nM) for 2 days and analyzed for H3K9me3 level in the FAS promoter region (ChIP 1 region as shown in Fig. 4A and B) by ChIP and semi-quantitative PCR. Right panel: SW620 cells were treated as in the left panel and analyzed for H3K9me3 level in the FAS promoter region by ChIP and real-time PCR. B. Left panel: SW480 and SW620 cells were treated with verticillin A for 2 days and analyzed for H3K9ac level in the FAS promoter region (ChIP 1 region as shown in Fig. 4A and B) by ChIP and semi-quantitative PCR. Right panel: SW480 cells were treated with verticillin A for 2 days and analyzed for H3K9ac level in the FAS promoter region by ChIP and real-time PCR C. SW620 cells were treated with verticillin A (50 nM) for 2 days and analyzed for RNA Pol II level in the FAS promoter region (ChIP 1 region as shown in Fig. 4A and B) by ChIP and semi-quantitative PCR. Right panel: SW620 cells were treated as in the left panel and analyzed for RNA Pol II level in the FAS promoter region by ChIP and real-time PCR.