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. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: J Immunol. 2015 Jul 1;195(4):1868–1882. doi: 10.4049/jimmunol.1402243

Figure 6. G9a SUV39H1 and SUV39H2 cooperate to mediate H3K9 trimethylation and FAS silencing.

Figure 6

A. SW620-5FU-R cells were transiently transfected with scramble, G9a-, SUV39H1-, and SUV39H2-specific siRNA (200 nM), respectively, for approximately 24h and analyzed for the respective mRNA levels as indicated by real time RT-PCR. The Fas mRNA level in scralble siRNA-transfected cells were arbitrarily set at 1. B. Cells as described in A were extracted for histone and analyzed by Western blotting using H3K9me3-specific antibody. H3 was used as normalization control. C. Cells as described in A were also analyzed for Fas expression levels using two Fas-specific PCR primer pairs [Fas 2 and Fas 3 (supplemental table 1)]. D. SW620-5FU-R cells were transiently transfected with scramble siRNA and pooled siRNAs of G9a SUV39H1 and SUV39H2 (200 nM each) for approximately 24h and analyzed for Fas expression using two pairs of Fas PCR primers and real-time RT-PCR.