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. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: J Immunol. 2015 Jul 15;195(4):1359–1363. doi: 10.4049/jimmunol.1500264

Figure 1.

Figure 1

Identification of AS-IL1α. (A) Circos plot showing differentially expressed genes in splenocytes from L. Monocytogenes infected mice. Chromosomes are indicated on the outer tracks. The middle track shows log2 fold-change values for all lncRNAs and AS-IL1α (colored red). The inner track shows log2 fold-change values for protein-coding genes, with immune genes colored in red. (B) AS-IL1α schematic in murine macrophages. AS-IL1α chromosomal localization overlaps with IL-1α protein coding gene on chromosome 2 indicated by vertical bar. (C) Macrophages infected with L. Monocytogenes for 3 hours at MOI 5 and MOI 10. Data were normalized with GAPDH, fold change calculated and are representative of 2 independent experiments (D) Polysome profiling of GAPDH, IL-1α and AS-IL1α transcripts. Immortalized macrophages were stimulated with LPS and either treated with cycloheximide alone, or harringtonine prior to cycloheximide treatment to determine translational potential of AS-IL1α.