A, B. Western blots and densitometric analysis representing phosphorylation of Akt in 13 week-old UCP-DTA and control hearts perfused with or without 1nM insulin (n=4). C–E. Western blots and densitometric analysis representing phosphorylation of Akt and GSK3 in 24 week-old UCP-DTA and control hearts with or without 1nM insulin. E, F. Fold increase of insulin stimulated phospho-Akt and GSK3 over basal (n=5). *p<0.05 vs. other genotypes in the same groups; # p<0.05 for insulin- stimulated vs. basal state for same genotype.
Saline-treated control wildtype (WT) mice, MnTBAP-treated WT mice, UCP-DTA treated with saline, UCP-DTA treated with MnTBAP.