Figure 1. Phenotying of blood monocyte and DC subsets in humans (A) and rhesus macaques (B).

EDTA-treated blood samples were stained with antibodies shown in Table 1 and analyzed by 11-color flow cytometry. (A) In HLA-DR⁺CD3⁻CD20⁻CD56⁻ populations, human monocyte and DC subsets were gated and divided into 4 populations by CD14 and CD16 expression as follows; (a) CD14⁺CD16⁻ monocytes, (b) CD14⁺CD16⁺ monocytes, (c) CD14⁻CD16⁺ monocytes, and a CD14⁻CD16⁻ population that was further divided into (d) CD123⁺ pDC and (e) BDCA-1⁺ mDC. In addition, a CD141⁺ mDC (f) was identified. (B) To analyze rhesus monocyte and DC subsets, HLA-DR⁺CD3⁻CD20⁻CD8⁻cell populations were similarly gated and further divided as described in panel A with the exception that antibody to human CD141 (BDCA-3) did not cross-react to, or detect this marker on rhesus macaque cells. The populations of cells identified included; (a) CD14⁺CD16⁻ monocytes, (b) CD14⁺CD16⁺ monocytes, (c) CD14⁻CD16⁺ monocytes, (d) CD123⁺ pDC, and (e) BDCA-1⁺ mDC.