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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Allergy Clin Immunol. 2015 Mar 4;136(2):295–303.e7. doi: 10.1016/j.jaci.2014.11.043

Figure 3. Increased presence of memory Th2 and Th17 cells in the lungs one month after coexposures to HDM and DEP.

Figure 3

(A) Th1, Th2 and Th17 lungs cells were identified by FACS as CD4+ T-cells expressing respectively IFNγ, IL13, and IL17A, respectively, following ex vivo stimulation with PMA/ionomycin for 4h. (B) Percentage of IL13+IL17+ double producers among CD4+CD44+ T-cells and (C) IL7 receptor mean fluorescence intensity (CD127 MFI) on naïve and CD4+CD44+ T-cells. (C) IL4 and IL17A were detected by ELISA in supernatant of lung cells stimulated with HDM (30μg/ml) for 5 days (n=4–8 mice/group; ***p<0.001, *p<0.05, 1-way ANOVA with Bonferroni’s multiple comparison test; p value calculated by T-test; n.s. = not significant).