Figure 4.

Deletion analysis of CLDN7 promoter activity. A: Representation of the pGL4C7 CLDN7/luciferase (luc) reporter. The sequence includes the transcription start site and the 5′ untranslated region (UTR) of CLDN7. B:CLDN7 promoter activity increases during intestinal epithelial cell (IEC) differentiation. C: Right: Truncated CLDN7 promoter luciferase reporter constructs were used to determine the minimal promoter region required for CLDN7 promoter activity during IEC differentiation. Left: Representation of the truncated CLDN7 luciferase reporters. Representative graphs of three independent experiments are shown for B and C.