Table 3.

Primers Used to Create CLDN7/Luciferase Reporters

Primer name	Sequence	Notes
Cloning primers for the initial CLDN7 construct
CLDN7 forward 2	5′-ATGGTACCAAGGAAAGTCAGTTGCGGTAG-3′	Cloning primer
CLDN7 reverse	5′-GTCACATGTTCCGCCCTCAGAAAACAG-3′	Cloning primer
CLDN7 mut forward	5′-GTGTTTTCTGAGGGCGGAAATGGAAGCTTAATGTTTTTGGCATCTTCCATTC-3′	Quickchange primer
CLDN7 mut reverse	5′-GAATGGAAGATGCCAAAAACATTAAGCTTCCATTTCCGCCCTCAGAAAACAC-3′	Quickchange primer
Cloning primers for deletion mutant CLDN7 luciferase constructs in the NcoI-SmaI region of the promoter
Luc2 ApaI reverse V	5′-GGCGCTGGGCCCTTCTTAAT-3′	Cloning primer at the ApaI site in the luc2 gene
Cl7 trunc forward 1	5′-ATATGGTACCTAGCCCTCACCCTGCTC-3′	Cloning primer 200 after the NcoI site
Cl7 trunc forward 2	5′-ATATGGTACCTGTAGCAGAGCCAGAGAAC-3′	Cloning primer 400 after the NcoI site
Cl7 trunc forward 3b	5′-ATATGGTACCAGGCGCACCTGTTGGGAA-3′	Cloning primer 625 after the NcoI site
Cl7 trunc forward 4	5′-ATATGGTACCTGGGCAAGGAGGGGTGG-3′	Cloning primer 801 after the NcoI site

Artificial KpnI (ATATGGTA) site is marked in bold at 5′ end.

mut, mutation.