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. 2015 Aug;185(8):2206–2218. doi: 10.1016/j.ajpath.2015.04.023

Table 4.

Primers for Megaprimer Mutagenesis of the CLDN7 Promoter

Primer name Sequence Use
CLDN7 SphI 5′-ATGCGCATGCTTTGAGCCCAGG-3′ With CLDN7 Mut1, produces Mut 1 Mega
CLDN7 Mut1 5′-CACCTCGACCTCCCAAAGTGGTCGGATTACAGGCGTGAG-3′ With CLDN7 SphI produces Mut 1 Mega
CLDN7 EcorI 5′-CCTCAGCCTCCCAAGTAGCT-3′ With Mut 1 Mega
CLDN7 forward 1 5′-CTCGGCCGGCTTTAGGTCCCAGTGGTTTC-3′ With CLDN7 Mut2, produces Mut2 Mega
CLDN7 Mut2 5′-TCCCCGCCGGGTTCTGTCCAAAGTGGTCTCTCCTCGGCCCTGCTT-3′ With CLDN7 F1, produces Mut 2 Mega
CLDN7 reverse 1 5′-GTCCCCGAAGGCCAGGCGGT-3′