Table 5.
Candidate TFs That Bind to the CLDN7 Promoter
| TF name | Fold | # TFBs | Accession number(s) |
|---|---|---|---|
| PU.1 | 3.9 | 1 | T02068 |
| ATF | 3.1 | 2 | T00051 |
| HNF-4α | 3.2 | 2 | T03828 |
| HNF-1C | 3.5 | 3 | T01951 |
| Sp1 | 2.4 | 4 | T00759 |
| GATA-1 | 2.6 | 5 | T00306 |
| ETF | 2.9 | 10 | T00270 |
| HOXD9/10 | 2.1 | 10 | T01424, T01425 |
| NF-κB/B1 | 2.3 | 10 | T00590, T00593 |
| c-Myb | 7.3 | 11 | T00137 |
| HNF-3α | −1.7 | 11 | T02512 |
| Elk-1 | 3.1 | 14 | T00250 |
| NF-1 | 2.3 | 28 | T00539 |
| AP-2αA | 8.0 | 34 | T00035 |
| Pax-5 | 3.2 | 51 | T00070 |
The DNA binding activity of nuclear lysates from differentiated and undifferentiated Caco-2 cells were compared using a TF/DNA array. Transcription factors (TFs) that showed differential DNA binding activity were cross-referenced with potential TF binding (TFB) sites on the CLDN7 promoter. Fold-change is the signal ratio of differentiated/nondifferentiated Caco-2 nuclear lysate on the TF/DNA binding array. Number of hits and TRANSFAC accession numbers (http://www.gene-regulation.com/pub/databases.html; last accessed April 4, 2015; registration required) are based on the in silico promoter analysis.