Supplemental Figure S4.

DLX4 induces IL-1β expression. Levels of IL-1β protein were assayed by enzyme-linked immunosorbent assay in lysates of vector-control (empty vector) and +DLX4 A2780 cells and in +DLX4 A2780 cells transfected with nontargeting shRNA (shControl) and IL1B shRNA (shIL1B) (A), vector-control and +DLX4 ES2 cells, and +DLX4 ES2 cells transfected with nontargeting and IL1B shRNAs (B), and control and DLX4-knockdown 2008 cells and DLX4-knockdown 2008 cells transfected with IL1B cDNA (shDLX4-A + IL1B) (C). Data are expressed as means ± SD for values of three independent assays. ^∗∗P < 0.01 versus empty vector; ^††P < 0.01 versus shControl. shDLX4-A, DLX4 shRNA.