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Immunogenicity of ascending LEISHDNAVAX doses in mice. Groups of 10 BALB/c mice (5 male, 5 female) were immunized i.d. at the tail base five times in weekly intervals with either PBS or LEISHDNAVAX (10, 50 or 100 μg per dose). Fourteen days after the last immunization LEISHDNAVAX-specific antibodies (total IgG) in sera were quantified by ELISA using plates coated with a mixture of recombinant LEISHDNAVAX antigens. ***P⩽0.001 (Student's t-test); n.s.: not significant.
