Figure 3.

Parasite burden in BALB/c and C57BL/6 mice following administration of single and repeated doses of LEISHDNAVAX and PBS. Parasite burden expressed in Leishman-Donovan Units (LDU) is shown for BALB/c mice in spleens (a) and livers (b) (n=8–9 mice per group) and for C57BL/6 mice in spleens (c) and livers (d) (n=4–8 mice per group). Mice were infected on day 0, treated once (day 7), twice (day 7 and 14) or three times (day 7, 14 and 21), respectively, with 100 μg LEISHDNAVAX per dose or PBS. Parasite burden was determined 7 days after the last treatment and in addition, for two groups of C57BL/6 mice receiving either three LEISHDNAVAX or PBS injections, 29 days after the third treatment (day 50 post infection). White bars represent PBS-treated groups and dark gray bars LEISHDNAVAX-treated groups, error bars indicate s.e.m. Differences between groups were statistically not significant based on a one-way ANOVA followed by Bonferroni's multiple comparison's test, which compared groups that had received the same number of injections of PBS or LEISHDNAVAX (P>0.05).