Table 7.
Screening of the bioremediation potential in the bacteria collection established from the sediment of El-Max district.
| Isolate | Biofilm | b.h. | ST (mN m−1) | Hydro (%) | Growth on different hydrocarbons | PCR amplification of gene markers | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Xyl | Oct | Pyr | DBT | Phe | Naph | Oil | nccA (a) | ACR3(2)(b) | phnA (c) | |||||
| S1-1 | 0.023 ± 0.0071 | − | 39.33 ± 3.05 | 38 ± 2.466 | + | + | + | − | + | + | + | − | + | − |
| S1-4 | 0.164 ± 0.0789 | − | 45.63 ± 0.55 | n.d. | + | + | + | + | + | + | + | − | + | − |
| S1-5 | 0.024 ± 0.0081 | − | 10.42 ± 1.64 | 5.9 ± 1.322 | − | + | − | − | − | − | + | − | − | + |
| S1-7 | 0.014 ± 0.0123 | + | 20.33 ± 1.52 | 5.1 ± 1.607 | − | + | − | + | − | − | + | − | + | − |
| S1-9 | 0.0125 ± 0.0071 | + | 32.33 ± 1.52 | 20.3 ± 2.020 | + | + | − | − | − | − | + | + | + | − |
| S1-10 | 0.0112 ± 0.0035 | − | 28.66 ± 1.52 | 46.2 ± 0.954 | − | − | − | − | − | − | + | + | + | − |
| S1-11 | 0.041 ± 0.0111 | + | 18.66 ± 0.57 | 10.4 ± 1.527 | − | + | − | − | − | − | + | + | + | − |
| S1-12 | 0.014 ± 0.0472 | − | 15.83 ± 0.76 | 40.8 ± 0.831 | − | + | − | + | − | − | + | − | + | + |
| S1-13 | 0.024 ± 0.0081 | + | 12.96 ± 0.55 | n.d. | − | + | − | − | − | + | + | − | + | − |
| S1-16 | 0.035 ± 0.0135 | − | 28.66 ± 0.57 | 21.98 ± 1.527 | − | + | − | − | − | − | + | +/− | − | + |
| S1-17 | 0.013 ± 0.0086 | + | 29.3 ± 1.57 | 9.3 ± 0.5 | − | + | − | + | − | − | + | +/− | + | − |
| S1-20 | 0.0145 ± 0.0092 | − | 25.23 ± 1.05 | n.d. | + | + | + | + | + | + | + | − | − | − |
| S1-21 | 0.418 ± 0.0920 | − | 28.36 ± 1.28 | 10.4 ± 1.261 | + | + | + | + | + | + | + | − | − | − |
| S1-22 | 0.0297 ± 0.0113 | − | 20.33 ± 0.57 | n.d. | + | + | − | − | − | − | + | − | − | − |
| S1-23 | 0.009 ± 0.0054 | + | 27.23 ± 0.68 | 7.5 ± 0.550 | + | + | − | − | − | − | + | − | − | − |
| S1-24 | 0.0126 ± 0.0034 | − | 23.66 ± 1.52 | n.d. | + | + | + | + | + | + | + | +/− | − | − |
| S1-26 | 0.0525 ± 0.0147 | + | 29.1 ± 0.85 | 13.3 ± 0.941 | + | + | + | − | − | + | + | − | + | − |
| S1-28 | 0.013 ± 0.0081 | − | 40.5 ± 1.32 | 16.3 ± 0.774 | − | + | − | − | − | − | + | − | + | − |
| S1-29 | 0.004 ± 0.0021 | + | 20.63 ± 3.05 | 14.2 ± 1.286 | − | + | − | − | + | + | + | − | − | + |
| S1-30 | 0.012 ± 0.0046 | + | 47.26 ± 0.57 | 21.3 ± 1.382 | + | + | − | − | − | − | + | − | + | − |
| S1-31 | 0.012 ± 0.0058 | − | 20.36 ± 1.15 | 13.8 ± 0.694 | − | + | − | − | − | − | + | − | − | − |
| S1-32 | 0.0183 ± 0.0078 | − | 40.26 ± 1.44 | 15.8 ± 0.390 | + | + | − | − | − | − | + | + | − | − |
| S1-33 | 0.004 ± 0.0015 | − | 40.83 ± 0.76 | 34.7 ± 0.375 | − | − | − | − | − | − | + | +/− | + | − |
| S1-34 | 0.0134 ± 0.007 | − | 15.16 ± 1.75 | 22.2 ± 2.450 | − | + | − | − | − | − | + | − | + | − |
| S1-36 | 0.024 ± 0.0081 | + | 20.63 ± 3.05 | n.d. | + | + | − | − | − | − | + | + | − | − |
| SCP1 | 0.011 ± 0.0075 | − | 47.16 ± 1.52 | n.d. | − | − | − | − | − | − | − | − | − | − |
| SCP2 | 0.028 ± 0.0053 | + | 51.25 ± 0.77 | 11.3 ± 1.734 | − | − | + | + | + | + | + | − | − | − |
| SCP3 | 0.417 ± 0.0156 | − | 48.30 ± 1.34 | 31.6 ± 1.443 | + | + | − | + | + | + | − | + | − | − |
| SCR1a | 0.046 ± 0.0122 | + | 53.50 ± 1.25 | 77.3 ± 2.508 | n.d. | − | − | − | − | − | + | − | − | − |
| SCR2a | 0.040 ± 0.0083 | + | 46.67 ± 0.84 | 26.8 ± 1.527 | − | − | − | − | + | − | − | − | − | − |
| SCR3a | 0.030 ± 0.0083 | − | 57.64 ± 0.50 | 23.5 ± 1.702 | − | − | + | − | + | + | − | − | − | − |
| SCS1 | 0.037 ± 0.0132 | − | 52.52 ± 0.77 | 10.2 ± 1.297 | − | − | − | − | − | + | − | − | − | − |
| SCS2 | 0.037 ± 0.0132 | − | 22.90 ± 1.48 | 23.6 ± 1.950 | + | − | + | + | + | + | − | − | − | − |
| SCS3 | 0.065 ± 0.0140 | + | 41.56 ± 1.05 | n.d. | + | + | − | + | − | − | ++ | − | − | − |
| SCS4 | 0.075 ± 0.0018 | + | 46.20 ± 1.00 | 2.7 ± 1.322 | − | − | + | + | − | − | − | − | − | − |
| SCS6 | 0.032 ± 0.0016 | − | 44.35 ± 0.70 | n.d. | − | + | + | + | n.d. | n.d. | + | − | − | − |
| SCuP1 | 0.022 ± 0.0106 | + | 50.95 ± 1.52 | 46.4 ± 2.466 | − | − | − | + | + | + | + | − | − | − |
| SCuQ1 | 0.081 ± 0.0240 | − | 52.30 ± 1.07 | 0.96 ± 0.076 | − | − | − | + | − | + | − | − | − | |
| SCuQ2 | 0.007 ± 0.0097 | − | 59.82 ± 0.53 | 28.8 ± 1.258 | − | − | − | − | − | − | − | − | − | − |
| SCuR1a | 0.049 ± 0.0131 | − | 59.70 ± 1.36 | 7.1 ± 1.294 | + | + | − | + | + | + | − | − | − | − |
| SCuR2a | 0.003 ± 0.0081 | − | 50.09 ± 1.51 | 6.7 ± 0.475 | − | − | − | − | − | − | − | − | − | − |
| SCuR3a | 0.086 ± 0.0116 | + | 59.40 ± 0.72 | 8.7 ± 1.189 | − | + | + | + | + | + | − | − | − | − |
| SCuR4a | 0.051 ± 0.0116 | + | 35.87 ± 0.93 | 29.1 ± 1.322 | + | + | + | + | − | − | + | − | − | − |
| SCuR5a | 0.022 ± 0.0097 | + | 38.30 ± 1.07 | 22.7 ± 1.875 | − | + | + | + | − | + | ++ | − | − | − |
| SC∗Q2 | 0.032 ± 0.0142 | + | 47.84 ± 1.11 | 9 ± 0.304 | − | − | − | + | − | + | − | − | − | − |
| SC∗Q3 | 0.026 ± 0.005 | − | 48.71 ± 2.23 | 3 ± 0.132 | n.d. | − | − | + | − | − | − | − | − | − |
| SC∗R2a | 0.177 ± 0.0512 | − | 55.60 ± 1.92 | 6.6 ± 0.25 | + | + | − | + | + | + | − | − | − | − |
| SC∗R3a | 0.366 ± 0.0361 | + | 42.6 ± 0.808 | n.d. | n.d. | n.d. | − | + | − | − | n.d. | − | − | − |
| SC∗S1 | 0.067 ± 0.0272 | − | 16.88 ± 0.76 | 36.6 ± 2.432 | − | − | − | − | + | + | ++ | − | − | − |
| SC∗S6 | 0.012 ± 0.0083 | − | 51.25 ± 1.86 | 18.3 ± 0.125 | − | − | − | − | − | − | − | − | − | − |
+: growth; −: no growth; n.d.: not detected.
Xyl: xylene; Oct: octane; Pyr: pyrene; DBT: dibenzothiophene; Phe: phenanthrene; Naph: naphthalene; oil: crude oil.
ST: surface tension; Hydro: hydrophobicity ratio (%); b.h.: blood haemolysis; biofilm: biofilm formation.