Table 2.

Names, accession number, and primer sequences used in the study.

	Accession number	Left sequence	Right sequence
Irs2	NM_001168633	AGGTGACACTATAGAATAAGGCACTGGAGCCTTAC	GTACGACTCACTATAGGGAGCAGCACTTTACTCTTTCAC
Kcnj11	NM_031358	AGGTGACACTATAGAATACTACTTCAGGCAAAACTCTG	GTACGACTCACTATAGGGAGAACTTTCCAATATTTCTTTT
Insr	NM_017071	AGGTGACACTATAGAATAAGCTGGAGGAGTCTTCAT	GTACGACTCACTATAGGGAAAGGGATCTTCGCTTT
Gck	NM_001270849	AGGTGACACTATAGAATAATCTTTTGCAACACTCAGC	GTACGACTCACTATAGGGATTGTTGGTGCCCAGA
Pklr	NM_012624	AGGTGACACTATAGAATATCGGAGGTGGAAATTG	GTACGACTCACTATAGGGACTCTGGGCCGATTTT
Prkcd	NM_133307	AGGTGACACTATAGAATATCAAGAACCACGAGTTCA	GTACGACTCACTATAGGGATCTTTCTGGAAGATGGTG
B2m∗	NM_012512	AGGTGACACTATAGAATAATGCTTGCAGAGTTAAACA	GTACGACTCACTATAGGGATGCATAAAATATTTAAGGTAAGA
Hprt1∗,#	NM_012583	AGGTGACACTATAGAATATCCTCATGGACTGATTATG	GTACGACTCACTATAGGGACTGGTCATTACAGTAGCTCTT
Mapk1	NM_053842	AGGTGACACTATAGAATACATTTTTGAAGAGACTGCTC	GTACGACTCACTATAGGGAAACTCTCTGGACTGAAGAAT
Prkcz	NM_022507	AGGTGACACTATAGAATACTTTAACAGGAGAGCGTACT	GTACGACTCACTATAGGGATATTGTCATGTTTCCGAGAT
Ikbkb	NM_053355	AGGTGACACTATAGAATACTTGAACTTAAAGCTGGTTC	GTACGACTCACTATAGGGAACATTTTACTGTTGTCAAAGAG
Kan(r)∗∗
Mtor	NM_019906	AGGTGACACTATAGAATATGGAACTTCGAGAGATGAG	GTACGACTCACTATAGGGATCACTTCAAACTCCACATAC
Actb∗	NM_031144	AGGTGACACTATAGAATAAACTACATTCAATTCCATCA	GTACGACTCACTATAGGGATAAAACGCAGCTCAGTAAC
Pik3r1	NM_013005	AGGTGACACTATAGAATACATCAGTATTGGCTTACG	GTACGACTCACTATAGGGATCATTTACTTCTTCCCTTGA

^∗Housekeeping genes. ^#Normalization gene. Underlined sequences are left and right universal left and right sequences (tags). ^∗∗Internal control supplied by Beckman Coulter Inc (Miami, FL, USA) as part of the GeXP kit. RT conditions were 48°C for 1 min; 37°C for 5 min; 42°C for 60 min; 95°C for 5 min and then hold at 4°C. PCR conditions were initial denaturation at 95°C for 10 min, followed by two-step cycles of 94°C for 30 sec and 55°C for 30 sec, ending in a single extension cycle of 68°C for 1 min.