Figure 2.

Therapeutic nTreg treatment induces multilineage chimerism at doses of 3, 1, and 0.5 × 10⁶ cells without cytoreduction. B6 BMT recipients were treated with different doses of recipient nTregs (■ 3 × 10⁶ cells, n = 4; ◆ 1 × 10⁶ cells, n = 8; ∆ 0.5 × 10⁶ cells, n = 6; ○ 0.1 × 10⁶ cells, n = 6). Donor (H-2D^d+) chimerism among leukocytes of T cell (CD4⁺ and CD8⁺), B cell (B220⁺), and the myeloid (Mac1⁺) lineage was assessed by flow cytometry of peripheral blood at multiple time points and is shown as mean percent (error bars indicate standard deviation). Data are representative for at least 3 independent experiments per group.