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. 2015 Aug 10;5:13170. doi: 10.1038/srep13170

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Copyright © 2015, Macmillan Publishers Limited

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(a) Differentially expressed miRNAs in tamoxifen-resistant breast cancer cells (TamR) and long-term estrogen-deprived breast cancer cells (LTED) compared to their parental MCF-7 cells. TamR and LTED were established from MCF-7 cells (see Materials and Methods). Small RNA libraries were constructed from MCF-7, TamR, and LTED cells and sequenced using the Illumina GAIIx. (b) Percentages of top 20 miRNAs abundantly expressed in MCF-7 and endocrine therapy resistant cells. Percentages in distinctive cell lines were determined by a read count for each indicated miRNA normalized to the total count of 94 selected miRNAs (>10 reads) listed in Supplementary Table S1.